• Tom Kenny | voiced | Bentley Whittman aka ‘Wizard’ | Curt Connors / Bentley Whittman aka ‘Wizard’ / Announcer / Sci-Fi Narrator | Cartoon Narrator / Curt Connors | Whirlwind / Announcer | Phone / Mr. Molskin / Curt Connors / Aries / Computer / Bob / Vulture / S.H.I.E.L.D. Agent | 16 Episodes |
• Steven Weber | voiced | Symbiote / Horror Narrator | Cab Driver / Trapster / Green Goblin | Trapster / Green Goblin | Shield Agent #1 / Iron Patriot | Trapster / Green Goblin | 9 Episodes |
• Tara Strong | voiced | Thundra / Party Girl / Girl #2 | Newscaster / Woman | Girl / Sandy / Excited Girl #1 | 8 Episodes |
• Matt Lanter | voiced | Klaw / Venom / Agent Venom | 8 Episodes |
• Clancy Brown | voiced | Taskmaster / Taskmaster | Coach Yeager | 8 Episodes |
• Dee Bradley Baker | voiced | Flint a.k.a ‘Sandman’ / Dr. Curt Conners / Dr. Curt Conners a.k.a The Lizard | 7 Episodes |
• Kevin Michael Richardson | voiced | Andy Cain Marko a.k.a ‘Juggernaut’ / Mac | Bulldozer | Worker #2 / Frankenstein’s Monster / Groot | 6 Episodes |
• Troy Baker | voiced | Loki / Loki | Master Dwarf | Frost Giant / Loki | Wili / Hawkeye / Hawkeye | Loki | 6 Episodes |
• Steve Blum | voiced | Wolverine | Man / Wolverine | Beetle | Man / Beetle / Ka-Zar | 5 Episodes |
• Fred Tatasciore | voiced | Hulk | 5 Episodes |
• Travis Willingham | voiced | Thor / Thor | Skurge | Wodin | 5 Episodes |
• Misty Lee | voiced | Girl #1 / Computer / Reporter / Squirrel Girl / Woman | 5 Episodes |
• Rob Paulsen | voiced | Batroc a.k.a ‘The Leaper’ | Lab Tech #1 / Batroc a.k.a ‘The Leaper’ | 4 Episodes |
• Greg Cipes | voiced | Robot / Cop / Midtown Kid | Excited Girl #2 | 4 Episodes |
• Chi McBride | voiced | Principal / Taurus Zodiac Agent / Thunderball | Worker #4 / New Yorker | 4 Episodes |
• Diedrich Bader | voiced | Kraven the Hunter | 4 Episodes |
• Drake Bell | voiced | Cop / Bully #2 / Waiter | 4 Episodes |
• Adrian Pasdar | voiced | Tony Stark a.k.a Iron Man | 4 Episodes |
• Eric Bauza | voiced | Michael Tan a.k.a Swarm / Amadeus Cho a.k.a Iron Spider | 4 Episodes |
• Christopher Daniel Barnes | voiced | Electro / Spyder-Knight | 3 Episodes |
• Jack Coleman | voiced | Doctor Strange | 3 Episodes |
• Phil LaMarr | voiced | Jarvis / Cloak | 3 Episodes |
• Roger Craig Smith | voiced | Captain America | 3 Episodes |
• Rick D. Wasserman | voiced | Alex a.k.a Rhino | 2 Episodes |
• Mark Hamill | voiced | Nightmare | Shao Lao / Arnim Zola | 2 Episodes |
• Maurice LaMarche | voiced | Doctor Doom | 2 Episodes |
• Bumper Robinson | voiced | Falcon | 2 Episodes |
• Laura Bailey | voiced | Black Widow | 2 Episodes |
• Ogie Banks | voiced | Agent / Bully #1 | 2 Episodes |
• Dave Boat | voiced | Thor / Lion-Headed Zodiak #1 | 2 Episodes |
• Ashley Eckstein | voiced | Dagger | 2 Episodes |