• Cathy Weseluck | voiced | Spike / Mayor of Ponyville / Flitter / Female Pegasus / Rumble / Cloudchaser / Berry Pinch / Truffle Shuffle / Minuette / Librarian / Paradise / Working Pony / Mayor Mare | 40 Episodes |
• Tabitha St. Germain | voiced | Mrs. Cup Cake / Princess Luna / Nightmare Moon / Granny Smith / Derpy Hooves / Mrs. Cake / Rose / Mr. Waddle / Cherry Berry / Linky Shoeshine / Surf / Tomato Vendor / Pound Cake / Cup Cake / Wild Fire / Fleur de Verre / Amber Waves / Castle / Olden Pony / Pony #1 / Cadet #2 / Cloudchaser / Wild Flower / Mail pony | 21 Episodes |
• Nicole Oliver | voiced | Princess Celestia / Cheerilee / Daisy Jo / Autumn Gem | 21 Episodes |
• Michelle Creber | voiced | Apple Bloom / Sweetie Belle - singing | 17 Episodes |
• Claire Corlett | voiced | Sweetie Belle | 14 Episodes |
• Madeline Peters | voiced | Scootaloo / Noi | 14 Episodes |
• Peter New | voiced | Big Macintosh / Hoity-Toity / Uptight pony | 12 Episodes |
• Lee Tockar | voiced | Snips / Gizmo / Meadow Song / Cherry vendor / Male Pegasus 2 / School Pony / Shopkeeper / Beaver Foreman | 8 Episodes |
• Brenda Crichlow | voiced | Zecora / Matilda | 7 Episodes |
• Richard Ian Cox | voiced | Snails / Mr. Greenhooves / Clump / Press Pony | 6 Episodes |
• Shannon Chan-Kent | voiced | Silver Spoon / Pinkie Pie / Vendor | 6 Episodes |
• Andrea Libman | voiced | Pumpkin Cake / Twinkleshine / Spectator #2 / Cadet #1 / Wild Flower | 5 Episodes |
• Chantal Strand | voiced | Diamond Tiara / Spectator #1 | 4 Episodes |
• Andrew Francis | voiced | Shining Armor / Lucky Clover / Royal Guards / Night Knight / Crystal Arrow | 4 Episodes |
• Britt McKillip | voiced | Princess Cadance / Lyra Heartstrings | 4 Episodes |
• Emilie-Claire Barlow | voiced | Cheerilee | 4 Episodes |
• Jayson Thiessen | voiced | Royal Guard / Bulk Biceps / Professor | 4 Episodes |
• Rebecca Shoichet | voiced | Twilight Sparkle | 4 Episodes |
• Kathleen Barr | voiced | Trixie / Queen Chrysalis / Trixie Lulamoon | 3 Episodes |
• Trevor Devall | voiced | Fancypants / Iron Will / Film Announcer / Thunderlane | 3 Episodes |
• Kazumi Evans | voiced | Rarity | 3 Episodes |
• John de Lancie | voiced | Discord | 2 Episodes |
• Ashleigh Ball | voiced | Dragon / Cloudchaser | 2 Episodes |
• Jimmy Miller (1) | voiced | King Sombra | 2 Episodes |
• Kelly Metzger | voiced | Spitfire | 2 Episodes |
• Vincent Tong | voiced | Garble / Donut Joe | 2 Episodes |